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Cloning and Expression
Enzymes
MscI
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MscI
MscI from New England Biolabs
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Citations:
(2)
Supplier Page
Supplier Page from
New England Biolabs for
MscI
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Description
Msc I (15,000 units/ml)
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
MscI
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
R0534M
Quantity
1250 units
Concentration
15,000 units/ml
Applications
DNA Manipulation
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Restriction Enzyme
Temperature
-20 C
Sequence
TGG/CCA
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New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (2)
(1)
DiMeLo-seq: a long-read, single-molecule method for mapping protein–DNA interactions genome wide
Nature Methods
June 1, 2022
Nicolas Altemose, Annie Maslan, Owen K. Smith, Kousik Sundararajan, Rachel R. Brown, Reet Mishra, Angela M. Detweiler, Norma Neff, Karen H. Miga, Aaron F. Straight,
et al.
solubilized. 37 μl
NEBuffer 2.1
was added, along with 100 units of
MscI
and 100 units of
AseI
(
NEB
#
R0534M
and #
R0526M
) to a total volume of 370 μl in a 1.5 ml lo-bind Eppendorf tube. This was placed on a rotator
(2)
OTX2 is a therapeutic target for retinoblastoma and may function as a common factor between C-MYC, CRX, and phosphorylated RB pathways
International Journal of Oncology
November 1, 2015
JING LI, CHUNHUI DI, JENNY JING, QUN DI, JONATHAN NAKHLA, DAVID CORY ADAMSON
to ligate the gene into pLVTHM (#12247, Addgene, Cambridge, MA, USA)
cloning
vector.
MscI
and
FspI
(#
R0534M
and #
R0135L
,
New England Biolabs
) were used to ligate it into the lentiviral construct pLVCT-tTR‑KRAB (#11643,
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MscI from New England Biolabs
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